Run the pipeline script directly
The pipeline at its core is just a python script, and you can run it on the command line for a single sample (see command-line usage), which you can also get on the command line by running
pipelines/pepatac.py --help. You just need to pass a few command-line parameters to specify sample name, reference genome, input files, etc. Here's the basic command to run a small test example through the pipeline:
pipelines/pepatac.py --single-or-paired paired \ --prealignments rCRSd human_repeats \ --genome hg38 \ --sample-name test1 \ --input examples/data/test1_r1.fastq.gz \ --input2 examples/data/test1_r2.fastq.gz \ --genome-size hs \ -O $HOME/pepatac_test
This example should take about 15 minutes to complete. See other example commands that use test data.