PBC2 (PCR Bottleneck Coefficient 2): one read pairs / two read pairs.
<sample_name>_peaks_chr_dist.[png/pdf]: Plot(s) of the chromosomal distribution of called peaks.
<sample_name>_<feature>_coverage.bed: A BED format file containing the number of overlapping reads in each <feature>.
<sample_name>_frif.[png/pdf]: Plot(s) of the fractions of reads in each <feature>. The cumulative sum of reads in a feature is plotted against the log of the number of bases covered by that feature. Therefore, it takes fewer total bases covered to visualize an enriched feature compared to others. Because peaks are called specifically on the data (i.e. enriched specifically for the data), the cumulative fraction of reads in peaks should generally be the first (left-most) curve. The number following the feature name is the fraction of reads in that feature. Features may be customized by specifying the --anno_name argument to the pipeline along with the corresponding BED file containing defined features.
<sample_name>_fragLen.txt: A text file containing a single column of all fragment lengths.
<sample_name>_fragCount.txt: A tabular file containing the number of read fragments at each fragment length.
<sample_name>_fragLenDistribution.txt: A tabular file containing fragment length distribution summary statistics.
<sample_name>_fragLenDistribution.[png/pdf]: Fragment length distribution plot(s). Normally, you should observe a well-defined peak somewhere <100-bp representing nucleosome-free regions, a second peak around 200-bp representing mono-nucleosomes, then sequentially smaller peaks representing multiple nucleosomes.
<sample_name>_peaks_partition_dist.[png/pdf]: Plot(s) of the number of peaks present in each defined genomic partition. Defined partitions include: 3' UTR, 5' UTR, Exonic, Intronic, Intergenic, Promoter, and Promoter Flanking Regions.
<sample_name>_peaks_TSS_dist.[png/pdf]: Plot(s) of the distribution of reads relative to TSS's.
<sample_name>_TssEnrichment.txt: A text file containing a single column of read counts at each position flanking transcription start sites (-2000 - TSS - +2000).
<sample_name>_TssEnrichment.[png/pdf]: Global TSS enrichment plot(s). Illustrates the aggregate enrichment around all transcription start sites.
The tool to produce these tracks, bamSitesToWig.py, is included in the PEPATAC repository and may be called indpendently with parameters like the --smooth-length further customized.
nucleotide-resolution ("exact cut") signal: The nucleotide-resolution (or "exact cut") signal is a signal track (bigWig) marking exact locations where the transposition event occurred. The Tn5 transpoase acts on and duplicates a 9-bp region, and the event location is defined as the center of this 9-bp window. To produce this signal, read positions on positive strands are shifted +4 with minus strand positions shifted -5.
smooth signal: The smooth signal track is produced alongside the nucleotide-resolution track. Instead of marking exact bases, the smooth track marks reads positioned +/- 25-bp around the "exact cut" location, to yield a 50-bp window centered on this position.
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